Parasite loads in local and non-local colonies over time (10 – 70 days after requeening) (A) Nosema spp. levels (spores per bee). Nosema load measurements commenced 13 days after requeen- ing the colonies and were measured at 47 and 68days post- requeening, for a total of three measurements. Fifty-bee samples were collected from the brood chamber of each colony.